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rabbit polyclonal anti-cyclin-dependent kinase inhibitor 1 (p21  (ImmunoWay Biotechnology Company)

 
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    ImmunoWay Biotechnology Company rabbit polyclonal anti-cyclin-dependent kinase inhibitor 1 (p21
    Knockdown of CDCA3 induces G0/G1-phase arrest in DU145 and PC-3 cells. (A) Flow cytometry was used to analyze the cell cycle following CDCA3 knockdown in DU145 and PC-3 cells. (B) Cell cycle-associated protein levels, including cyclin D1 and <t>p21,</t> were analyzed via western blotting. GAPDH was used as a loading control. Data are presented as the mean ± SD of three independent experiments. **P<0.01 and ***P<0.001. CDCA3, cell division cycle-associated 3; sh-, short hairpin; NC, negative control.
    Rabbit Polyclonal Anti Cyclin Dependent Kinase Inhibitor 1 (P21, supplied by ImmunoWay Biotechnology Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti-cyclin-dependent+kinase+inhibitor+1+(p21/p21+antibody/pmc08759108-91-36-49
    Average 90 stars, based on 1 article reviews
    rabbit polyclonal anti-cyclin-dependent kinase inhibitor 1 (p21 - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "Suppression of CDCA3 inhibits prostate cancer progression via NF-κB/cyclin D1 signaling inactivation and p21 accumulation"

    Article Title: Suppression of CDCA3 inhibits prostate cancer progression via NF-κB/cyclin D1 signaling inactivation and p21 accumulation

    Journal: Oncology Reports

    doi: 10.3892/or.2021.8253

    Knockdown of CDCA3 induces G0/G1-phase arrest in DU145 and PC-3 cells. (A) Flow cytometry was used to analyze the cell cycle following CDCA3 knockdown in DU145 and PC-3 cells. (B) Cell cycle-associated protein levels, including cyclin D1 and p21, were analyzed via western blotting. GAPDH was used as a loading control. Data are presented as the mean ± SD of three independent experiments. **P<0.01 and ***P<0.001. CDCA3, cell division cycle-associated 3; sh-, short hairpin; NC, negative control.
    Figure Legend Snippet: Knockdown of CDCA3 induces G0/G1-phase arrest in DU145 and PC-3 cells. (A) Flow cytometry was used to analyze the cell cycle following CDCA3 knockdown in DU145 and PC-3 cells. (B) Cell cycle-associated protein levels, including cyclin D1 and p21, were analyzed via western blotting. GAPDH was used as a loading control. Data are presented as the mean ± SD of three independent experiments. **P<0.01 and ***P<0.001. CDCA3, cell division cycle-associated 3; sh-, short hairpin; NC, negative control.

    Techniques Used: Flow Cytometry, Western Blot, Negative Control

    Related Articles

    Flow Cytometry:

    Article Title: Suppression of CDCA3 inhibits prostate cancer progression via NF-κB/cyclin D1 signaling inactivation and p21 accumulation
    Article Snippet: was used as the loading control. .. The following antibodies were used: Mouse monoclonal anti-GAPDH (1:2,000; cat. no. 33033M; BIOSS), rabbit polyclonal anti-CDCA3 (1:1,000; cat. no. YT0819), rabbit polyclonal anti-cleaved caspase-3 (1:1,000; cat. no. YC0006), rabbit polyclonal anti-pro-caspase-3 (1:1,000; cat. no. YT6113), and rabbit polyclonal anti-cyclin-dependent kinase inhibitor 1 (p21; 1:1,000; cat. no. YT3497; all from Immunoway Biotechnology Company), rabbit polyclonal anti-cyclin D1 (1:1,000; cat. no. 0623R; BIOSS), mouse monoclonal anti-NFκB-p65 (1:1,000; cat. no. YM311), rabbit polyclonal anti-phosphorylated (p)-NFκB-p65 (1:1,000; cat. no. YP0192), rabbit polyclonal anti-IKKα/β (1:1,000; cat. no. YT2302), and rabbit polyclonal anti-NFκB-p105/p50 (1:1,000; cat. no. YT3101; all from Immunoway Biotechnology Company), HRP-labeled goat anti-rabbit secondary antibody (1:5,000; cat. no. 40295G-HRP; BIOSS) and HRP-labeled goat anti-mouse secondary antibody (1:5,000; cat. no. 0368G-HRP; BIOSS).

    Western Blot:

    Article Title: Suppression of CDCA3 inhibits prostate cancer progression via NF-κB/cyclin D1 signaling inactivation and p21 accumulation
    Article Snippet: was used as the loading control. .. The following antibodies were used: Mouse monoclonal anti-GAPDH (1:2,000; cat. no. 33033M; BIOSS), rabbit polyclonal anti-CDCA3 (1:1,000; cat. no. YT0819), rabbit polyclonal anti-cleaved caspase-3 (1:1,000; cat. no. YC0006), rabbit polyclonal anti-pro-caspase-3 (1:1,000; cat. no. YT6113), and rabbit polyclonal anti-cyclin-dependent kinase inhibitor 1 (p21; 1:1,000; cat. no. YT3497; all from Immunoway Biotechnology Company), rabbit polyclonal anti-cyclin D1 (1:1,000; cat. no. 0623R; BIOSS), mouse monoclonal anti-NFκB-p65 (1:1,000; cat. no. YM311), rabbit polyclonal anti-phosphorylated (p)-NFκB-p65 (1:1,000; cat. no. YP0192), rabbit polyclonal anti-IKKα/β (1:1,000; cat. no. YT2302), and rabbit polyclonal anti-NFκB-p105/p50 (1:1,000; cat. no. YT3101; all from Immunoway Biotechnology Company), HRP-labeled goat anti-rabbit secondary antibody (1:5,000; cat. no. 40295G-HRP; BIOSS) and HRP-labeled goat anti-mouse secondary antibody (1:5,000; cat. no. 0368G-HRP; BIOSS).

    Negative Control:

    Article Title: Suppression of CDCA3 inhibits prostate cancer progression via NF-κB/cyclin D1 signaling inactivation and p21 accumulation
    Article Snippet: was used as the loading control. .. The following antibodies were used: Mouse monoclonal anti-GAPDH (1:2,000; cat. no. 33033M; BIOSS), rabbit polyclonal anti-CDCA3 (1:1,000; cat. no. YT0819), rabbit polyclonal anti-cleaved caspase-3 (1:1,000; cat. no. YC0006), rabbit polyclonal anti-pro-caspase-3 (1:1,000; cat. no. YT6113), and rabbit polyclonal anti-cyclin-dependent kinase inhibitor 1 (p21; 1:1,000; cat. no. YT3497; all from Immunoway Biotechnology Company), rabbit polyclonal anti-cyclin D1 (1:1,000; cat. no. 0623R; BIOSS), mouse monoclonal anti-NFκB-p65 (1:1,000; cat. no. YM311), rabbit polyclonal anti-phosphorylated (p)-NFκB-p65 (1:1,000; cat. no. YP0192), rabbit polyclonal anti-IKKα/β (1:1,000; cat. no. YT2302), and rabbit polyclonal anti-NFκB-p105/p50 (1:1,000; cat. no. YT3101; all from Immunoway Biotechnology Company), HRP-labeled goat anti-rabbit secondary antibody (1:5,000; cat. no. 40295G-HRP; BIOSS) and HRP-labeled goat anti-mouse secondary antibody (1:5,000; cat. no. 0368G-HRP; BIOSS).



    Similar Products

    rabbit polyclonal anti-cyclin-dependent kinase inhibitor 1 (p21  (ImmunoWay Biotechnology Company)
    90
    ImmunoWay Biotechnology Company rabbit polyclonal anti-cyclin-dependent kinase inhibitor 1 (p21
    Knockdown of CDCA3 induces G0/G1-phase arrest in DU145 and PC-3 cells. (A) Flow cytometry was used to analyze the cell cycle following CDCA3 knockdown in DU145 and PC-3 cells. (B) Cell cycle-associated protein levels, including cyclin D1 and <t>p21,</t> were analyzed via western blotting. GAPDH was used as a loading control. Data are presented as the mean ± SD of three independent experiments. **P<0.01 and ***P<0.001. CDCA3, cell division cycle-associated 3; sh-, short hairpin; NC, negative control.
    Rabbit Polyclonal Anti Cyclin Dependent Kinase Inhibitor 1 (P21, supplied by ImmunoWay Biotechnology Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti-cyclin-dependent+kinase+inhibitor+1+(p21/p21+antibody/pmc08759108-91-36-49
    Average 90 stars, based on 1 article reviews
    rabbit polyclonal anti-cyclin-dependent kinase inhibitor 1 (p21 - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Knockdown of CDCA3 induces G0/G1-phase arrest in DU145 and PC-3 cells. (A) Flow cytometry was used to analyze the cell cycle following CDCA3 knockdown in DU145 and PC-3 cells. (B) Cell cycle-associated protein levels, including cyclin D1 and p21, were analyzed via western blotting. GAPDH was used as a loading control. Data are presented as the mean ± SD of three independent experiments. **P<0.01 and ***P<0.001. CDCA3, cell division cycle-associated 3; sh-, short hairpin; NC, negative control.

    Journal: Oncology Reports

    Article Title: Suppression of CDCA3 inhibits prostate cancer progression via NF-κB/cyclin D1 signaling inactivation and p21 accumulation

    doi: 10.3892/or.2021.8253

    Figure Lengend Snippet: Knockdown of CDCA3 induces G0/G1-phase arrest in DU145 and PC-3 cells. (A) Flow cytometry was used to analyze the cell cycle following CDCA3 knockdown in DU145 and PC-3 cells. (B) Cell cycle-associated protein levels, including cyclin D1 and p21, were analyzed via western blotting. GAPDH was used as a loading control. Data are presented as the mean ± SD of three independent experiments. **P<0.01 and ***P<0.001. CDCA3, cell division cycle-associated 3; sh-, short hairpin; NC, negative control.

    Article Snippet: The following antibodies were used: Mouse monoclonal anti-GAPDH (1:2,000; cat. no. 33033M; BIOSS), rabbit polyclonal anti-CDCA3 (1:1,000; cat. no. YT0819), rabbit polyclonal anti-cleaved caspase-3 (1:1,000; cat. no. YC0006), rabbit polyclonal anti-pro-caspase-3 (1:1,000; cat. no. YT6113), and rabbit polyclonal anti-cyclin-dependent kinase inhibitor 1 (p21; 1:1,000; cat. no. YT3497; all from Immunoway Biotechnology Company), rabbit polyclonal anti-cyclin D1 (1:1,000; cat. no. 0623R; BIOSS), mouse monoclonal anti-NFκB-p65 (1:1,000; cat. no. YM311), rabbit polyclonal anti-phosphorylated (p)-NFκB-p65 (1:1,000; cat. no. YP0192), rabbit polyclonal anti-IKKα/β (1:1,000; cat. no. YT2302), and rabbit polyclonal anti-NFκB-p105/p50 (1:1,000; cat. no. YT3101; all from Immunoway Biotechnology Company), HRP-labeled goat anti-rabbit secondary antibody (1:5,000; cat. no. 40295G-HRP; BIOSS) and HRP-labeled goat anti-mouse secondary antibody (1:5,000; cat. no. 0368G-HRP; BIOSS).

    Techniques: Flow Cytometry, Western Blot, Negative Control